DIY Enzymes & Reagents
- Recombinant p50-T4 DNA Ligase
- Barrick Lab’s pfu-sso7d (Phusion Polymerase)
- dCas9 (Nuclease-dead S. pyogenes Cas9)
- T7 RNA Polymerase (IVT Workhorse)
- Biotin Ligase (BirA, in vitro biotinylation)
- Forbidden Technique: Bootleg Coomassie Blue
- Pipette Jockey’s Homebrew Reverse Transcriptase (Mashup-RT)
Dreams From the Wet Lab
- The Definitive Dank Plasmid Name Tier List
- Forbidden Technique: Productive Procrastination
- Is RPMI 1640 cherry flavored?
- The Great PCR Test
- Tales of Green Fluorescence (GFPs of Wrath) or The Trouble with Mastermixes
- The Whiteboard Desk Experiment
- Of Flow Sorts and Cohorts
Explanations & Protocols
- Zen and the Art of Flow Sorting
- Denaturing / Alkaline DNA Gel Electrophoresis
- Flow Cytometry Panel Design
- Hyper-efficient DNA EtOH Precipitation
- R21 Writing Guide
Philosophical Musings
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Featured
The Definitive Dank Plasmid Name Tier List
All placements are scientific fact; dispute at the risk of being a science denier. Plasmids are rated on these factors to determine their rank: (1) Dankness, (2) Applicability of name to vector function.
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Featured
The Art of Corner Cutting
The “Winging It” strategy is the 9th circle of Hell (Treachery) in Dante’s Inferno. You will find yourself frozen in place by gusts of chaos and uncertainty generated by what will seem to be a literal demon. First, realize the benefits of the situation…
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Handbook for Pure Science
When I started working in a lab, I thought being clever was enough. “Each variable in an experiment is a Chess piece,” I told myself, “and the successful experiment is nothing more than moving the pieces in the correct way, at the correct time.” …
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Chemically Incompetent 