DIY Enzymes & Reagents
- Recombinant p50-T4 DNA Ligase
- Barrick Lab’s pfu-sso7d (Phusion Polymerase)
- dCas9 (Nuclease-dead S. pyogenes Cas9)
- T7 RNA Polymerase (IVT Workhorse)
- Biotin Ligase (BirA, in vitro biotinylation)
- Forbidden Technique: Bootleg Coomassie Blue
- Pipette Jockey’s Homebrew Reverse Transcriptase (Mashup-RT)
Dreams From the Wet Lab
- Forbidden Technique: Productive Procrastination
- Is RPMI 1640 cherry flavored?
- The Great PCR Test
- Tales of Green Fluorescence (GFPs of Wrath) or The Trouble with Mastermixes
- The Whiteboard Desk Experiment
- Of Flow Sorts and Cohorts
Explanations & Protocols
- Zen and the Art of Flow Sorting
- Denaturing / Alkaline DNA Gel Electrophoresis
- Flow Cytometry Panel Design
- Hyper-efficient DNA EtOH Precipitation
Philosophical Musings
- The Art of Corner Cutting
- The Constituent Elements of Wisdom
- How to Train Your Undergrad
- Teaching and the Absurd Analogy
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Featured
The Art of Corner Cutting
The “Winging It” strategy is the 9th circle of Hell (Treachery) in Dante’s Inferno. You will find yourself frozen in place by gusts of chaos and uncertainty generated by what will seem to be a literal demon. First, realize the benefits of the situation…
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Zen and the Art of Fluorescence-activated Cell Sorting (FACS)
It’s Time to Evolve Your Consciousness The day has finally come: you’re tired of sitting at the microscope with your Hunchback of Notre Dame lookin’ posture, haemocytometer, cell counter, and trypan blue in hand, clicking away. You look down at your ice box, unable to pull your eyes away from the source of the subconscious… Read more
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